Abstract
Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced GLP-1 release from EECs is modulated by serotonin through a process involving serotonin receptor interaction. This was studied by assessing the effects of serotonin reuptake inhibition by fluoxetine on nutrient-induced GLP-1, PYY and CCK release from isolated pig intestinal segments. Next, serotonin-induced GLP-1 release was studied in enteroendocrine STC-1 cells, where effects of serotonin receptor inhibition were studied using specific and non-specific antagonists.Casein (1% w/v), safflower oil (3.35% w/v), sucrose (50 mM) and rebaudioside A (12.5 mM) stimulated GLP-1 release from intestinal segments, whereas casein only stimulated PYY and CCK release. Combining nutrients with fluoxetine further increased nutrient-induced GLP-1, PYY and CCK release.Serotonin release from intestinal tissue segments was stimulated by casein and safflower oil while sucrose and rebaudioside A had no effect. The combination with fluoxetine (0.155 μM) further enhanced casein and safflower oil induced-serotonin release.Exposure of ileal tissue segments to serotonin (30 μM) stimulated GLP-1 release whereas it did not induce PYY and CCK release. Serotonin (30 and 100 μM) also stimulated GLP-1 release from STC-1 cells, which was inhibited by the non-specific 5HT receptor antagonist asenapine (1 and 10 μM). These data suggest that nutrient-induced GLP-1 release is modulated by serotonin through a receptor mediated process.
| Original language | English |
|---|---|
| Pages (from-to) | 142-150 |
| Journal | Journal of Nutritional Biochemistry |
| Volume | 32 |
| DOIs | |
| Publication status | Published - 2016 |
Keywords
- GLP-1
- Nutrients
- Rebaudioside A
- Serotonin
- Small intestine
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Ripken, D., van der Wielen, N., Wortelboer, H. M., Meijerink, J., Witkamp, R. F., & Hendriks, H. F. J. (2016). Nutrient-induced glucagon like peptide-1 release is modulated by serotonin. Journal of Nutritional Biochemistry, 32, 142-150. https://doi.org/10.1016/j.jnutbio.2016.03.006
Ripken, Dina ; van der Wielen, Nikkie ; Wortelboer, Heleen M. et al. / Nutrient-induced glucagon like peptide-1 release is modulated by serotonin. In: Journal of Nutritional Biochemistry. 2016 ; Vol. 32. pp. 142-150.
@article{f981ae20fd384f5b9e0cd6522d62c4ed,
title = "Nutrient-induced glucagon like peptide-1 release is modulated by serotonin",
abstract = "Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced GLP-1 release from EECs is modulated by serotonin through a process involving serotonin receptor interaction. This was studied by assessing the effects of serotonin reuptake inhibition by fluoxetine on nutrient-induced GLP-1, PYY and CCK release from isolated pig intestinal segments. Next, serotonin-induced GLP-1 release was studied in enteroendocrine STC-1 cells, where effects of serotonin receptor inhibition were studied using specific and non-specific antagonists.Casein (1% w/v), safflower oil (3.35% w/v), sucrose (50 mM) and rebaudioside A (12.5 mM) stimulated GLP-1 release from intestinal segments, whereas casein only stimulated PYY and CCK release. Combining nutrients with fluoxetine further increased nutrient-induced GLP-1, PYY and CCK release.Serotonin release from intestinal tissue segments was stimulated by casein and safflower oil while sucrose and rebaudioside A had no effect. The combination with fluoxetine (0.155 μM) further enhanced casein and safflower oil induced-serotonin release.Exposure of ileal tissue segments to serotonin (30 μM) stimulated GLP-1 release whereas it did not induce PYY and CCK release. Serotonin (30 and 100 μM) also stimulated GLP-1 release from STC-1 cells, which was inhibited by the non-specific 5HT receptor antagonist asenapine (1 and 10 μM). These data suggest that nutrient-induced GLP-1 release is modulated by serotonin through a receptor mediated process.",
keywords = "GLP-1, Nutrients, Rebaudioside A, Serotonin, Small intestine",
author = "Dina Ripken and {van der Wielen}, Nikkie and Wortelboer, {Heleen M.} and Jocelijn Meijerink and Witkamp, {Renger F.} and Hendriks, {Henk F.J.}",
year = "2016",
doi = "10.1016/j.jnutbio.2016.03.006",
language = "English",
volume = "32",
pages = "142--150",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier",
}
Ripken, D, van der Wielen, N, Wortelboer, HM, Meijerink, J, Witkamp, RF & Hendriks, HFJ 2016, 'Nutrient-induced glucagon like peptide-1 release is modulated by serotonin', Journal of Nutritional Biochemistry, vol. 32, pp. 142-150. https://doi.org/10.1016/j.jnutbio.2016.03.006
Nutrient-induced glucagon like peptide-1 release is modulated by serotonin. / Ripken, Dina; van der Wielen, Nikkie; Wortelboer, Heleen M. et al.
In: Journal of Nutritional Biochemistry, Vol. 32, 2016, p. 142-150.
Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Nutrient-induced glucagon like peptide-1 release is modulated by serotonin
AU - Ripken, Dina
AU - van der Wielen, Nikkie
AU - Wortelboer, Heleen M.
AU - Meijerink, Jocelijn
AU - Witkamp, Renger F.
AU - Hendriks, Henk F.J.
PY - 2016
Y1 - 2016
N2 - Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced GLP-1 release from EECs is modulated by serotonin through a process involving serotonin receptor interaction. This was studied by assessing the effects of serotonin reuptake inhibition by fluoxetine on nutrient-induced GLP-1, PYY and CCK release from isolated pig intestinal segments. Next, serotonin-induced GLP-1 release was studied in enteroendocrine STC-1 cells, where effects of serotonin receptor inhibition were studied using specific and non-specific antagonists.Casein (1% w/v), safflower oil (3.35% w/v), sucrose (50 mM) and rebaudioside A (12.5 mM) stimulated GLP-1 release from intestinal segments, whereas casein only stimulated PYY and CCK release. Combining nutrients with fluoxetine further increased nutrient-induced GLP-1, PYY and CCK release.Serotonin release from intestinal tissue segments was stimulated by casein and safflower oil while sucrose and rebaudioside A had no effect. The combination with fluoxetine (0.155 μM) further enhanced casein and safflower oil induced-serotonin release.Exposure of ileal tissue segments to serotonin (30 μM) stimulated GLP-1 release whereas it did not induce PYY and CCK release. Serotonin (30 and 100 μM) also stimulated GLP-1 release from STC-1 cells, which was inhibited by the non-specific 5HT receptor antagonist asenapine (1 and 10 μM). These data suggest that nutrient-induced GLP-1 release is modulated by serotonin through a receptor mediated process.
AB - Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced GLP-1 release from EECs is modulated by serotonin through a process involving serotonin receptor interaction. This was studied by assessing the effects of serotonin reuptake inhibition by fluoxetine on nutrient-induced GLP-1, PYY and CCK release from isolated pig intestinal segments. Next, serotonin-induced GLP-1 release was studied in enteroendocrine STC-1 cells, where effects of serotonin receptor inhibition were studied using specific and non-specific antagonists.Casein (1% w/v), safflower oil (3.35% w/v), sucrose (50 mM) and rebaudioside A (12.5 mM) stimulated GLP-1 release from intestinal segments, whereas casein only stimulated PYY and CCK release. Combining nutrients with fluoxetine further increased nutrient-induced GLP-1, PYY and CCK release.Serotonin release from intestinal tissue segments was stimulated by casein and safflower oil while sucrose and rebaudioside A had no effect. The combination with fluoxetine (0.155 μM) further enhanced casein and safflower oil induced-serotonin release.Exposure of ileal tissue segments to serotonin (30 μM) stimulated GLP-1 release whereas it did not induce PYY and CCK release. Serotonin (30 and 100 μM) also stimulated GLP-1 release from STC-1 cells, which was inhibited by the non-specific 5HT receptor antagonist asenapine (1 and 10 μM). These data suggest that nutrient-induced GLP-1 release is modulated by serotonin through a receptor mediated process.
KW - GLP-1
KW - Nutrients
KW - Rebaudioside A
KW - Serotonin
KW - Small intestine
U2 - 10.1016/j.jnutbio.2016.03.006
DO - 10.1016/j.jnutbio.2016.03.006
M3 - Article
AN - SCOPUS:84962873386
SN - 0955-2863
VL - 32
SP - 142
EP - 150
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
ER -
Ripken D, van der Wielen N, Wortelboer HM, Meijerink J, Witkamp RF, Hendriks HFJ. Nutrient-induced glucagon like peptide-1 release is modulated by serotonin. Journal of Nutritional Biochemistry. 2016;32:142-150. doi: 10.1016/j.jnutbio.2016.03.006
